Regarding fasta file

[huma-cloud]

Hello Everyone!

I've a some confusions,

1. I've upto 200 samples in my VCF file and I wanna convert a specific gene's position to protein sequence from VCF file. The confusion is: should I use the FASTA sequence as full-genomic.fa (introns+exons) or just need to use CDS.fa?
2. And one more thing, should i need to enter only 1 reference genomic/CDS.fa for my gene; or I need to write 200 genomic/CDS.fa according to my number of samples in my VCF file?

Please answer my query!!!!!
Thanks in advance!