Main tool: pod5
Code repository: https://github.com/nanoporetech/pod5-file-format
Additional tools:
- python 3.9.17
Basic information on how to use this tool:
- executable:
pod5 - help:
--help - version:
--version - description: "The pod5 package provides the following tools for inspecting and manipulating POD5 files as well as converting between .pod5 and .fast5 file formats."
Additional information:
Full documentation: https://pod5-file-format.readthedocs.io/en/latest/index.html
# split pod5 files into channel-specific pod5 files for parallelization of dorado basecalling
pod5 view . --include "read_id, channel" --output summary.tsv && \
pod5 subset . --summary summary.tsv --columns channel