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FLIMPA version 1.4

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@SofiaKapsiani SofiaKapsiani released this 01 Oct 20:17
· 1 commit to main since this release

New updates

  • Baseline Correction Control:

    • Added the parameter Baseline correction to allow users to control whether baseline correction is performed. If enabled, baseline corrections removes constant electronic (DC) noise from the fluorescence decay curve, which improves Fourier Transform analysis.
    • Added % time bins (baseline corr.) parameter to enable users to specify the percentage of initial time bins used for the Baseline correction
  • .ptu Artifact Handling:

    • To prevent inaccurate results caused by excessive time-binning, if a binning factor greater than 2 is detected in a $\text{.ptu}$ file, FLIMPA automatically sets Baseline correction to False. This is due to time binning causes the early time points (which FLIMPA assumes to only contain constant DC noise) to be grouped with the fluorescence signal, leading to inaccurate baseline correction.
  • Reference File :

    • Users can now select the same file for both data analysis and reference correction.
    • Reference files are automatically renamed with an _reference suffix (e.g., [original name]_reference).
  • Frequency:

    • The Frequency (MHz) parameter now supports floating-point values (e.g., 80.08 MHz)